Characterization of Embryonic Stem-Like Cell Lines Derived from Embryoid Bodies.
نویسندگان
چکیده
منابع مشابه
Establishing Clonal Cell Lines with Endothelial-Like Potential from CD9hi, SSEA-1− Cells in Embryonic Stem Cell-Derived Embryoid Bodies
BACKGROUND Differentiation of embryonic stem cells (ESCs) into specific cell types with minimal risk of teratoma formation could be efficiently directed by first reducing the differentiation potential of ESCs through the generation of clonal, self-renewing lineage-restricted stem cell lines. Efforts to isolate these stem cells are, however, mired in an impasse where the lack of purified lineage...
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BioTechniques 349 Embryonic stem (ES) cells were established from the inner cell mass of blastocysts and have been shown to spontaneously differentiate into multiple cell types representative of all three embryonic germ layers, both in teratomas after grafting or in vitro under appropriate conditions (1,2). ES cells can be maintained in their pluripotent state if cultured in the presence of leu...
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Embryonic stem cells (ESC) have been established previously from the inner cell mass cells of mouse blastocysts. In suspension culture, they spontaneously differentiate to blood-island-containing cystic embryoid bodies (CEB). The development of blood vessels from in situ differentiating endothelial cells of blood islands, a process which we call vasculogenesis, was induced by injecting ESC into...
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With their unique ability to differentiate into all cell types, embryonic stem (ES) cells hold great therapeutic promise. To improve the efficiency of embryoid body (EB)-mediated ES cell differentiation, we studied murine EBs on the basis of their size and found that EBs with an intermediate size (diameter 100-300 microm) are the most proliferative, hold the greatest differentiation potential, ...
متن کاملTGF-β1 induces the formation of vascular-like structures in embryoid bodies derived from human embryonic stem cells
Human embryonic stem cells (ESCs) can differentiate into endothelial cells in response to stimuli from extracellular cytokines. Transforming growth factor (TGF)-β1 signaling is involved in stem cell renewal and vascular development. Previously, human ESCs were isolated from inner cell mass and a stable ESC line was developed. In the present study, the effects of extracellular TGF-β1 were invest...
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ژورنال
عنوان ژورنال: Experimental Animals
سال: 1997
ISSN: 1341-1357,1881-7122
DOI: 10.1538/expanim.46.11